When talking about traditional medicine from the Amazon, Ayahuasca is usually the first thing that comes to mind. However, in the jungle there are numerous indigenous groups with different dialects, beliefs, worldviews and ancestral healing practices. Although it may seem incredible, many of these groups do not know the use of Ayahuasca, the best-known master plant.
Among these groups are the Matsés ethnic, belonging to the Pano linguistic family. While the Matsés do not use Ayahuasca, they have other lesser-known practices, such as using the glandular secretion of the monkey frog (Phyllomedusa bicolor). Originally called Kampu or Acate in the Matsés language, this practice has gained popularity in the West under the name Kambô.

Kambô, also known as frog medicine, toad medicine, toad vaccine, or jungle vaccine, is an ancestral tradition practiced by native Matsés experts and other Amazonian ethnic groups, primarily from the Pano linguistic family.
This traditional medicine is used to boost energy, enhance self-confidence, and improve hunting skills. After receiving Kambô treatment, the Matsés head out to hunt, showcasing their finest archery skills. Users of Kambô report that it not only boosts their energy and stamina but also heightens sensory perceptions and enhances mental clarity and tranquility, thereby improving their overall hunting prowess.
Traditional Use
The practice of Kambô begins with the capture of a frog of the species Phyllomedusa bicolor, also known as the giant monkey frog or the bicolor tree frog.
A Matsés expert immobilizes the frog by carefully tying its four extremities, provoking the frog to release its secretion as a defense mechanism. The expert then collects the secretion from the frog’s skin and places it on a specially prepared piece of wood designed to contain and preserve the substance. Afterwards, the frog is released back into the jungle.

Before applying Kambô, the expert burns the end of a thin vine, known locally as tamshi, and uses it to create small, superficial, circular burns on the skin, causing blisters to form. The number of burns depends on the individual’s tolerance. The expert then carefully removes the skin from the blisters and applies the Kambô substance to the exposed wounds, allowing the secretion to enter the body. It is essential for the person to drink plenty of fluids before receiving Kambô.
In men, Kambô is typically applied to the arm or chest, while in women it is applied to the arm or abdomen. Due to its analgesic properties, Matsés experts also use Kambô on areas of the body experiencing pain.
After application, the experience is usually intense for about 15 minutes, with effects such as profuse sweating, nausea, and vomiting. Following this period, the individual enters a state of deep relaxation.

This practice, often conducted on rainy days, is also considered useful for ‘purifying the body’.
Scientific Studies
Currently, scientific research on Kambô secretion is in its early stages, and further studies are needed to fully understand its effects and efficacy in treating various medical conditions.
One of the early pioneers in the scientific exploration of the Phyllomedusa bicolor frog secretion was the renowned Italian pharmacologist Vittorio Erspamer in the 1960s. His research uncovered the presence of bioactive peptides in the frog’s secretion with potent pharmacological properties, including:
- Cardiovascular Effects: Dilation of blood vessels, reduction in blood pressure, and an increase in heart rate.
- Analgesic Effects: Pain relief by inhibiting the transmission of pain signals in the nervous system.
- Anti-Inflammatory Effects: Reduction of inflammation through modulation of the immune system.
- Antimicrobial Effects: Elimination or inhibition of microorganism growth.
Subsequent research has identified specific peptides in the secretion, such as deltorphin and dermorphin. Deltorphin acts as an emetic, while dermorphin is recognized for its powerful analgesic properties.
Additional studies suggest the potential therapeutic benefits of Phyllomedusa bicolor frog secretion for treating conditions like depression, arthritis, infections, inflammatory diseases, hypertension, and other cardiovascular issues.
While Kambô has traditionally been used within indigenous practices, it is increasingly being explored in alternative medicine due to its profound benefits for physical and mental well-being.
It is crucial to emphasize that research is still in its early phases.













